RotiGrow and other Instant Algae Feed Protocols

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N-Rich Protocols

Three Proven Enrichment protocols

Enrichment with rotifers grown using RotiGrow Plus

1. “Standard 8-hour enrichment”:

• Feed N-Rich Standard continuously with a peristaltic pump, or every hour for 6-8 hours.
• Feed rate of 13 ml per hour per 100g wet weight of rotifers (85 million rotifers at 0.18ng dry weight per rotifer).

2. “Quick (1-2 hour) enrichment” with optional continuing maintenance feeding:

• Feed N-Rich Plus at a rate of 18.5 ml per hour per 100g wet weight of rotifers. Feed for 1-2 hours to achieve desired level of enrichment.
• Continue Feeding N-Rich Standard at a rate of 5.5 ml per hour per 100g wet weight of rotifers to maintain rotifer health and enrichment for 8 hours or more.

Enrichment with rotifers grown on RotiGrow Nanno or Nanno 3600

3. “Quick (2-3 hour) enrichment” with optional continuing maintenance feeding:

• Feed N-Rich Nanno at a rate of 18.5 ml per hour per 100g wet weight of rotifers. Feed for 2-3 hours to achieve desired level of enrichment.
• Continue Feeding N-Rich Standard at a rate of 5.5 ml per hour per 100g wet weight of rotifers to maintain rotifer health and enrichment for 8 hours or more.

Note: It is possible to use N-Rich Plus or N-Rich Nanno for up to 8 hours for extremely high HUFA levels of up to 8% rotifer dry weight. Such enrichment is more stressful, but can be successful with additional monitoring of dissolved oxygen.